An ISFET Microarray Sensor System for Detecting the DNA Base Pairing

An Integrated ISFET Sensor Array

A monolithically integrated ISFET sensor array and interface circuit are described. A new high-density, low-power source-drain follower was developed for the sensor array. ISFETs were formed by depositing Au/Ti extended-gate electrodes on standard MOSFETs, then thin silicon nitride layers using catalytic chemical vapor deposition and/or SU-8 protective layers were formed on the extended-gate el...

Molecular electrostatic potentials of DNA base-base pairing and mispairing.

An understanding of why adenine (A) pairs with thymine (T) and cytosine (C) with guanine (G) in DNA is very useful in the design of sensors and other related devices. We report the use of dissociation energies, geometries and molecular electrostatic potentials (MEPs) to justify the canonical (AT and CG) Watson-Crick pairs. We also analyze all mismatches in both configurations-cis and trans-with...

Wireless electrochemical DNA microarray sensor.

We report an electrochemical DNA microarray sensor whose function is controlled with just two wires regardless of the number of individual sensing electrodes. The bipolar sensing electrode is modified with probe DNA, and the anode end of each electrode is configured to emit light (electrogenerated chemiluminescence) upon hybridization of cDNA labeled with electrocatalytic (oxygen reduction) Pt ...

6-Pyrazolylpurine as an Artificial Nucleobase for Metal-Mediated Base Pairing in DNA Duplexes

The artificial nucleobase 6-pyrazol-1-yl-purine (6PP) has been investigated with respect to its usability in metal-mediated base pairing. As was shown by temperature-dependent UV spectroscopy, 6PP may form weakly stabilizing 6PP-Ag(I)-6PP homo base pairs. Interestingly, 6PP can be used to selectively recognize a complementary pyrimidine nucleobase. The addition of Ag(I) to a DNA duplex comprisi...

An Intelligent System for Automated DNA Base Calling